{"product_id":"cell-free-gene-expression-methods-and-protocols-9781071620007","title":"Cell-Free Gene Expression: Methods and Protocols","description":"\u003cp\u003e\u003cb\u003ePart I: Tools for Cell-Free Expression Systems\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1. Best Practices for DNA Template Preparation towards Improved Reproducibility in Cell-Free Protein Production\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Eugenia F. Romantseva, Drew S. Tack, Nina Alperovich, David Ross, and Elizabeth A. Strychalski\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. Simple Extract Preparation Methods for \u003ci\u003eE. coli-\u003c\/i\u003eBased Cell-Free Expression\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Alissa C. Mullin, Taylor Slouka, and Javin P. Oza\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. Preparation and Screening of Cell-Free Extract from Non-Growing \u003ci\u003eEscherichia coli \u003c\/i\u003eA19\u003ci\u003e \u003c\/i\u003eCells\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Florian Hiering, Jurek Failmezger, and Martin Siemann-Herzberg\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Cell-Free Protein Synthesis Using \u003ci\u003ePichia pastoris\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Alex J. Spice, Rochelle Aw, and Karen M. Polizzi\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. A Streptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Huiling Xu, Wan-Qiu Liu, and Jian Li\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Hossein Moghimianavval, Yen-Yu Hsu, Alessandro Groaz, and Allen P. Liu\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Conary Meyer, Chuqing Zhou, Zecong Fang, Marjorie L. Longo, Tingrui Pan, and Cheemeng Tan\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. Cell-Free Gene Expression from DNA Brushes\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Michael Levy, Ohad Vonshak, Yiftach Divon, Ferdinand Greiss, Noa Avidan, Shirley S. Daube, and Roy H. Bar-Ziv\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Kazuaki Amikura, Keita Hibi, and Yoshihiro Shimizu\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. Measurement of Transcription, Translation, and Other Enzymatic Processes during Cell-Free Expression Using PERSIA\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Scott Wick and Peter A. Carr\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II: Applications of Cell-Free Expression Systems\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. Cell-Free Noncanonical Redox Cofactor Systems\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e William B. Black and Han Li\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Blake J. Rasor, Bastian Vögeli, Michael C. Jewett, and Ashty S. Karim\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e13. Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e April M. Miguez, Yan Zhang, and Mark P. Styczynski\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e14. Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Orion M. Venero, Wakana Sato, Joseph M. Heili, Christopher Deich, and Katarzyna P. Adamala\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e15. Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Imre Banlaki, François-Xavier Lehr, and Henrike Niederholtmeyer\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Ashty S. Karim\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 01\/07\/2023\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 435\u003cbr\u003e\u003cb\u003eBinding Type:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.73lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.92d\u003cbr\u003e\u003cb\u003eISBN:\u003c\/b\u003e 9781071620007","brand":"Humana","offers":[{"title":"Paperback","offer_id":40833128366195,"sku":"9.78107E+12","price":237.72,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0555\/9255\/0515\/products\/img_d053465a-e4ff-4783-a9e1-44e09d3357c8.jpg?v=1684846536","url":"https:\/\/bookstorenmore.com\/products\/cell-free-gene-expression-methods-and-protocols-9781071620007","provider":"Bookstore N More","version":"1.0","type":"link"}