Candida Species: Methods and Protocols

Part I: The Candida Genome

1. CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans

Ben A. Evans and Douglas A. Bernstein

2. Plasmid-Based CRISPR-Cas9 Editing in Multiple Candida Species

Lisa Lombardi and Geraldine Butler

3. A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans

Raju Shivarathri, Manju Chauhan, Rounik Mazumdar, Phan Canh Trinh, Wolfgang Reiter, Markus Hartl, Karl Kuchler, and Neeraj Chauhan

4. How to Use the Candida Genome Database

Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock

5. Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata

Eunice López-Fuentes, Grecia Hernández-Hernández, Alejandro De Las Peñas, and Irene Castaño

6. Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans

Virginia Glazer and Damian Krysan

Part II: Antifungal Discovery and Resistance

7. Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance

Jane Usher

8. Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS)

Jessica Regan and Glen E. Palmer

9. High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis

Mohit Kumar, Rajendra Prasad, and Ashutosh Singh

10. Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library

Lulu Zhang, Dongmei Li, Richard Calderone, and Xiaodong She

11. Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans

Dongmei Li, Xiaodong She, and Richard Calderone

Part III: Pathogenesis and Virulence

12. In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin

Sejeong Lee, Nessim Kichik, Olivia W. Hepworth, Jonathan P. Richardson, and Julian R. Naglik

13. Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors

Liesbeth Demuyser, Wouter Van Genechten, and Patrick Van Dijck

14. Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy

Junko Yano and Paul L. Fidel, Jr.

15. A Simple Method for Growth of Candida albicans B

Author: Richard Calderone
Publisher: Humana
Published: 08/26/2022
Pages: 390
Binding Type: Hardcover
Weight: 2.02lbs
Size: 10.00h x 7.00w x 0.94d
ISBN: 9781071625484

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