Engineering Natural Product Biosynthesis: Methods and Protocols

1. A bioinformatics workflow for investigating fungal biosynthetic gene clusters

Jorge C. Navarro-Muñoz and Jérôme Collemare

2. Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression - Aspergillus oryzae as a heterologous host

Kate M. J. de Mattos-Shipley, Colin M. Lazarus and Katherine Williams

3. Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Aspergillus nidulans as a Heterologous Host

Danielle A. Yee and Yi Tang

4. Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Fusarium sp. as a Heterologous Host

Mikkel Rank Nielsen, and Jens Laurids S rensen

5. Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans using episomal vectors

Indra Roux and Yit Heng Chooi

6. Targeted Genetic Engineering via Agrobacterium-mediated transformation in Fusarium solani

Mikkel Rank Nielsen, Samba Evelyne Kabemba Kaniki, and Jens Laurids S rensen

7. Investigating Fungal Biosynthetic Pathways Using Pichia pastoris as a Heterologous Host

Zhilan Qian, Qi Liu, and Menghao Cai

8. Evolutionary genome mining for the discovery and engineering of natural product biosynthesis

Marc G. Chevrette, Nelly Selem-Mojica, César Aguilar, Kristin Labby, Edder D. Bustos-Diaz, Jo Handelsman, and Francisco Barona-Gómez

9. Inducing Global Expression of Actinobacterial Biosynthetic Gene Clusters

Meghan Pepler, Xiafei Zhang, Hindra, and Marie A. Elliot

10. Engineering modular polyketide biosynthesis in Streptomyces using CRISPR/Cas: a practical guide

Jean-Malo Massicard, Li Su, Christophe Jacob and Kira J Weissman

11. CRISPR/Cas9-Based Methods for Inactivating Actinobacterial Biosynthetic Genes and Elucidating Function

Audam Chhun and Fabrizio Alberti

12. Understanding and Manipulating Assembly Line Biosynthesis by Heterologous Expression in Streptomyces

Lihan Zhang, Takayoshi Awakawa, and Ikuro Abe

13. Heterologous expression, purification, and characterization of type II polyketide acyl carrier proteins

Grayson S. Hamrick, Casey H. Londerganm, and Louise K. Charkoudian

14. Cyanobacterial Genome Sequencing, Annotation and Bioinformatics

Jonna Teikari, Martin Baunach, and Elke Dittmann

15. Single crossover to inactivate target gene in cyanobacteria

Jaimie Gibbons, Liping Gu, Yeyan Qiu and Ruanbao Zhou

16. Double crossover approach to inactivate target gene in cyanobacteria

Jaimie Gibbons, Liping Gu and Ruanbao Zhou

17. Expression of Cyanobacterial Biosynthetic Gene Clusters in Escherichia coli

Alescia Cullen, Matthew Jordan, and Brett A. Neilan

18. Saccharomyces cerevisiae as a heterologous host for natural products

Maximilian Otto, Dany Liu, and Verena Siewers

19. Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host

Shanhui Xu, Sheng Wu, and Yanran Li

20. Rapid Combinatorial Co-Expression of

Author: Elizabeth Skellam
Publisher: Humana
Published: 06/07/2022
Pages: 488
Binding Type: Hardcover
Weight: 2.33lbs
Size: 10.00h x 7.00w x 1.06d
ISBN: 9781071622728

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