Long Non-Coding RNAs in Cancer

PART I: LNCRNA CLASSIFICATION AND UTILITY IN CANCER

1. LncRNAs and Available Databases

Sara Napoli

2. LncRNA as Cancer Biomarkers

Ingram Iaccarino and Wolfram Klapper

PART II: LNCRNA PROFILING AND DISCOVERY

3. Bioinformatics Pipeline to Analyze lncRNA Arrays

Katia Todoerti, Domenica Ronchetti, Martina Manzoni, Elisa Taiana, Antonino Neri, and Luca Agnelli

4. Bioinformatic Pipelines to Analyze lncRNAs RNAseq Data

Luca Agnelli, Stefania Bortoluzzi, and Giancarlo Pruneri

5. Single Cell RNAseq Analysis of lncRNAs

Stefano Cagnin, Enrico Alessio, Raphael Severino Bonadio, and Gabriele Sales

PART III: VALIDATION OF IDENTIFIED LNCRNAS

6. Measuring lncRNA Expression by Real Time PCR

Sonam Dhamija and Manoj B. Menon

7. Quantitation of Long Non-coding RNA using Digital PCR

Yu Ota, Irene K Yan, and Tushar Patel

8. Detection of lncRNA by LNA-based In Situ Hybridization in Paraffin Embedded Cancer Cell Spheroids

Boye Schnack Nielsen, Jesper Larsen, Jakob H ffding, Son Ly Nhat, Natasha Helleberg Madsen, Trine M ller, Bj rn Holst, Kim Holmstr m

PART IV: FUNCTIONAL STUDIES TO DECIPHER THE ROLE OF IDENTIFIED LNCRNAS

9. In vitro Silencing of lncRNA Expression using siRNAs

Meike Thijssen, Jennifer Bintz, and Luis Arnes

10. In vitro silencing of lncRNAs using LNA gapmeRs

Elisa Taiana, Vanessa Favasuli, Domenica Ronchetti, Eugenio Morelli, Pierfrancesco Tassone, Giuseppe Viglietto, Nikhil C. Munshi, Antonino Neri, and Nicola Amodio

11. Methods used to make Lipid Nanoparticles to deliver LNA Gapmers against lncRNAs into Acute Myeloid Leukemia (AML) Blasts

Chun-Tien Kuo, Robert Lee, and Ramiro Garzon

12. CRISPR/Cas9 to Silence Long Non-coding RNAs

Ingrid Arctander Rosenlund, George A. Calin, Mihnea P. Dragomir, and Erik Knutsen

13. CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) to explore the oncogenic lncRNA network

Eugenio Morelli, Annamaria Gulla', Nicola Amodio, Elisa Taiana, Antonino Neri, Mariateresa Fulciniti, and Nikhil C. Munshi

14. In vivo Silencing/Overexpression of lncRNAs by CRISPR/Cas System

Marianna Vitiello, Laura Poliseno, and Pier Paolo Pandolfi

15. Experimental Validation of the Non-Coding Potential for lncRNAs

Emily Dangelmaier and Ashish Lal

16. Identification of lncRNA-protein interactions by CLIP and RNA pull-down assays

Kunming Zhao, Xingwen Wang, and Ying Hu

17. Empirical Validation of Overlapping Virus lncRNAs and Coding Transcripts by Northern Blot

Mehmet Kara and Scott A. Tibbetts

PART V: LNCRNA ANALYSIS IN LIQUID BIOPSY

18. Phospho-RNAseq profiling of extracellular mRNAs and lncRNAs

Maria D. Giraldez and Muneesh Tewari

19. Detection of Circulating RNA using Nanopore Sequencing

Jennifer Lindemann, Irene K Yan, and Tushar Patel

20. LncRNA Quantification from Extracellular Vesicles Isolated from Blood Plasma or Conditioned Media

Joan J. Castellano, Jordi Canals, Bing Han, Tania Díaz, Mariano Monzo, and Alfons Navarro

PART VI: CIRCULAR RNAS

21. The use of circRNAs as Biomarkers of Cancer

Carla Solé, Gartze Mentxaka, and Charles H. Lawrie

22. Bioinformatic Analysis of Circular RNA Expression

Enrico Gaffo, Alessia Buratin, Anna Dal Molin, and Stefania Bortoluzzi

23. Study of circular RNAs (circRNAs) expression

Eleonora D'Ambra and Mariangela Morlan

Author: Alfons Navarro
Publisher: Humana
Published: 07/08/2022
Pages: 404
Binding Type: Paperback
Weight: 1.54lbs
Size: 10.00h x 7.00w x 0.83d
ISBN: 9781071615836

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